摘要
目的 探讨人类卵巢卵泡刺激素 (folliclestimulatinghormone,FSH) β基因表达。 方法 通过RT -PCR技术 ,分离、扩增人类卵巢组织FSHβ基因cDNA ,并进一步克隆、测序。 结果 测序结果与垂体组织克隆FSHβcDNA序列相一致。结论 人类卵巢组织自身转录FSHβ,提示有必要深入开展垂体 -性腺轴的基因表达谱研究。
Objective To investigate the expression of FSHβ in human ovary.Methods The RNA was isolated and purified from the tissue of human ovary.Then RT-PCR was performed with the primers of encoding the β-subunit of human follicle-stimulating hormone(hFSH β).The cDNA of hFSHβ was further cloned and sequenced.Results The cDNA sequence of β-subunit of hFSH in ovary was similar to that in pituitary.Conclusion Human ovary can self-express the hormone-specific β-subunit of FSH.It is necessary to further investigate the gene expression map of pituitary-sexual gland axis.
出处
《中国实用妇科与产科杂志》
CAS
CSCD
北大核心
2001年第12期731-732,共2页
Chinese Journal of Practical Gynecology and Obstetrics
基金
教育部高等学校骨干教师基金 ( 2 0 0 0 -6 5 )
海南省百项农业新技术基金 ( 1998-14 9)资助项目
