摘要
目的 探讨高原缺氧条件下 ,急性放射损伤小鼠存活率和造血系统的变化特点。方法 小鼠模拟 5 0 0 0m高原缺氧 3d后 ,接受 6Gy一次性γ射线全身照射 ,照后继续在缺氧条件下饲养 ,观察其存活率。外周血指标、骨髓有核细胞数、血清TGF β1 和EPO的含量、CFU GM和CFU E集落形成能力的改变、实验动物血清对CFU GM和CFU E集落形成的影响。结果 发现模拟高原缺氧条件下可提高急性放射损伤小鼠的存活率 ;并在照射后第 3天 ,表现为外周血红细胞和骨髓红系造血损伤较平原照射损伤为轻 ,但外周血白细胞和骨髓粒系造血损伤则有所加重。结论 模拟高原缺氧条件 。
Objective To explore the response of survival rate and the characteristics of hematopoietic system to acute radiation injury at high altitude. Methods A total of 180 mice were randomly and equally divided into 4 groups, that is, plain control group (C), plain + radiation group (R), high altitude group (H) and high altitude + radiation group (HR). After 3 d at plain or the simulated high altitude of 5 000 m, the rats of the 2 radiation groups were inflicted with 6 Gy total body irradiation of γ ray from 60 Co . The rats were kept living at plain or the simulated high altitude, and their survival rate was evaluated for 18 d. Blood cell count, Hb concentration, bone marrow nucleated cell (BMC) count, serum TGF β 1 and EPO concentrations, and the effects of serum on CFU E and CFU GM were determined in half of the animals 3 d after radiation. Results The survival rate of mouse with acute radiation injury was higher in those at the simulated high altitude 5 000 m than those at plain. On the 3rd day after radiation, the mice of HR group had higher RBC and Hb counts and better erythropoiesis, but lower WBC and BMC count and worse granulopoiesis in comparison with those of R group. Conclusion Simulated high altitude can protect the erythropoiesis of mice against acute radiation injury.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2001年第12期1450-1452,共3页
Journal of Third Military Medical University
关键词
高原缺氧
急性放射损伤
存活率
造血
小鼠
simulated high altitude
acute radiation injury
survival rate
hematopoietic
mice
