摘要
目的 通过观察肺癌癌组织、对应癌旁组织和远癌肺组织中细胞凋亡及p73基因转录表达水平的变化 ,探讨细胞凋亡及p73基因转录表达水平与肺癌发生、发展的关系。方法 采用TUNEL及RT PCR技术检测 3 2例非小细胞肺癌癌组织、癌旁组织和 7例肺良性病变组织 ,以及对应正常肺组织细胞凋亡和p73mRNA的表达水平 ,并结合临床病理学资料进行统计学分析。结果 ①肺癌组织中的凋亡指数明显高于癌旁肺组织和远癌肺组织 (P <0 0 1 ) ,细胞凋亡水平与肺癌组织学类型、分化程度和P TNM分期无明显关系 (P >0 0 5 ) ;②肺癌组织中p73mRNA表达水平和表达率明显高于癌旁肺组织、远癌肺组织和肺良性病变组织 (P <0 0 1 ) ;③组织中凋亡指数的升高和p73基因转录表达水平的增加呈显著的正相关 (P <0 0 1 )。
Objective To explore the relationship of the p73 gene transcript expression and the apoptosis level in cancerous tissues with the incidence and development of human non small cell lung cancer(NSCLC). Methods The expression of p73 mRNA and the apoptotic index (AI) was detected in 32 human NSCLC tissues, 32 para cancer tissues, 7 lung benign lesion tissues and matched non cancer lung tissues with RT PCR and TUNEL technique. All data were treated with statistical analysis. Results ① The AI was significantly higher in the lung cancer tissues than in the para cancer tissues and normal lung tissues ( P <0.01). No correlation was found between apoptosis level and the types, differentiation and P TNM stages of NSCLC. ② p73 was over expressed in 28/32 NSCLC tissues. The expression level of the p73 mRNA was significantly higher in the cancer tissues than in the para cancer tissues and benign lesion tissues ( P <0.01).③The expression of p73 gene was positively correlated with the AI of tissue ( P <0.01). Conclusion There is an over expression of p73 mRNA and increase of apoptosis level in NSCLC tissues.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2001年第12期1433-1435,共3页
Journal of Third Military Medical University
基金
重庆市应用基础基金资助项目 (99 5 6 5 8) )
