摘要
目的 了解HIV-1感染者体内中和抗体状况,探讨HIV-1感染的病理机制。方法 病毒的分离和培养采用共培养（co-culture）方法,从29例HIV-1感染者的外周血淋巴细胞（PBMC）分离了29株HIV-1原代病毒,中和实验采用病例血浆中和分离的相应病毒株,再感染经PHA刺激活化后的正常人PBMC培养5天检测其HIV-1p24抗原含量以判断中和效果。结果 29例研究对象中,24例是无症状感染者,5例为AIDS患者。我们发现17例无症状感染者的ID50≥8,其中10例ID90≥8,4例产生高滴度中和抗体ID90≥64,交叉中和实验结果表明这4例感染者的血浆对异体病毒均有不同程度的中和效应,其中尤以s7,s28作用最强。而AIDS患者中仅2例ID50≥8,其中1例ID90≥8无1例产生高滴度中和抗体。结论 通过对HIV-1感染者血浆中和效应的观察,表明多数感染者对自身同血源病毒的中和抗体滴度较低,但也有少数感染者能产生较高滴度的中和抗体,有必要深入研究其广泛的中和病毒效应;此外,早期无症状的感染者似比晚期有症状者对自体病毒的中和效应强,提示抗病毒治疗应于感染早期进行,以免机体体液免疫反应的进一步恶化。
Objective Neutralizing antibodies may be important for inhibiting HIV - 1 replication, and consequently an effective HIV- 1 preventive vaccine should be used to elicit neutralizing antibody responses. To search for possible epitopes for neutralizing antibodies, we assessed the neutralizing activity of sera of HIV - 1 infected individuals. Methods Twenty nine(6 females and 23 males)HIV- 1 infected individuals were enrolled in this study with an age ranging from 22 to 66 years. None of the subjects received combined therapy before virus isolation. Using co- culture technique, we were able to isolate 29 primary virus strains from their PBMC, one isolate from each infected individual. Virus neutralization was assessed by using primary isolates of HIV - 1 with phytohemagglutinin - stimulated PBMC as target cells and p24 antigen output as a measurement of virus production. Results Out of 29 subjects,24 were asymptomatic, and 5 were symptomatic. We found that there was more neutralization of autologous primary HIV - 1 isolates in sera of asymptomatic subjects( AS) than in those of symptomatic ones(SS) .The ID50 of 17 ASs were ≥8, only 2 SSs were≥8. The ID90 of ten ASs were ≥8, but among SSs only one was so. Moreover, of the neutralizing sera, 4 ASs were even ≥64, but none for SPs. The result of cross - neutralization experiment showed that these 4 ASs also had some broadly neutralizing activity. Conclusion This study show that the neutralizing activity from the sera of asymptomatic HIV - 1 infected indivicduals is stronger than from those of symptomatic individuals. Thus, if antiviral therapy is to be applied in these patients, it should be conducted earlier in order to protect the humoral immunity from being exhausted. In addition, such broadly neutralizing sera may be further explored for vaccine development tailored to Chinese population.
出处
《中国性病艾滋病防治》
2001年第4期202-205,共4页
Chinese Journal of Std & Aids Prevention and Control
关键词
HIV-1
中和抗体
艾滋病
体液免疫
HIV-1 (human immunodeficiency virus type 1) Neutralizing antibody AIDS (Acquired Immune De-ficiency Syndrome) Humoral immunology
