摘要
本文以海藻酸钙包埋法制备固定化恶臭假单胞菌细胞,以分批反应、间歇反应及连续柱式反应转化0.3mol/l 丙烯晴溶液来生产丙烯酰胺.丙烯晴溶液用0.05mol/l tris—HCl 缓冲液调至pH 值8.0,控制温度为5℃.间歇反应在恒温水浴摇床上以100r/min 振荡进行,13h 后丙烯酰胺浓度积累为30%,丙烯晴的转化率在95%以上.连柱柱式反应在带夹套的串联柱式反应器中进行,以每克湿固定化细胞每小时1 ml 的流速输入底物溶液,可有95%以上的丙烯晴被转化.酶柱实测半衰期达1200 h 以上.反应产物经分离提取后得到白色针状晶体,经红外光谱等鉴定,确认它为丙烯酰胺.
Pseudomonas putida JP-1 cells that possess high nitrile hydratase
activities were immobilized by being entrapped in calcium alginate gel and
crosslinked with glutaraldehyde.The conditions for the production of acryl-
amide with such immobilized cells were investigated with three methods:
batch process,semi-cotinuous process and column-continuous process.
The results showed that the optimal pH and temperature for the produ-
ction of acrylamide were 8.0 and 5℃,respectively;0.3mol/l acrylonitrile
in 0.05mol/l Tris-HCl buffer was the most suitable substrate concentration.
with the semi-continuous method,——the rocking rate being 100r/min,
and the column-continuous process,in a special series of columns at 150ml/h,
300 grams/liter of acrylamide was accumulated.The conversion rate of acr-
ylonitrile was more than 95%,and the halflife of immobilized cells was
more than 1200 h.
The crystal obtained from the product of reaction was identified as acr-
ylamide by means of gas chromatography-mass spectrometry IR spectrum and
other means.
出处
《上海交通大学学报》
EI
CAS
CSCD
北大核心
1991年第5期80-86,共7页
Journal of Shanghai Jiaotong University
基金
中国石化总公司资助
关键词
丙烯酰胺
恶臭假单胞菌
固定化
immobilized cells
pseudomonas putida
acrylamide
