摘要
目的 探讨混合酸脱钙时间及脱钙温度对切片质量的影响。方法 通过脱钙 ,记录脱钙时间、温度 ,制作石蜡切片 ,HE染色 ,显微镜观察等步骤 ,比较混合酸的脱钙时间及脱钙温度对切片质量造成的影响。结果 室温越高 ,脱钙时间越短 ,染色效果越好。结论 室温时 ,最佳脱钙时间为 8～ 9d ,选择合适的混合酸 。
Objective To investingate the effect of decalcific time and decalcific temperature of mixed acid on section quality. Methods To compare the effect of decalcific time and decalcific temperature of mixed acid on section quality by decalcifying and by recording the steps of decalcific time, decalcific temperature, paraffin section making, HE Staining,microscope observation,ect. Results The higher of the indoor temperature, the shorter of the decalcific time and the better of the staining. Conclusion The best decalcific time is 8～9 days on indoor temperature.
出处
《临床口腔医学杂志》
2001年第4期286-287,共2页
Journal of Clinical Stomatology
关键词
颌骨
石蜡切片
混合酸
脱钙
狗
jaw
paraffin section
mixed acid
decalcify
