摘要
目的 : 研究锌对红细胞膜离子转运功能的影响。方法 : 分体内和体外实验。雄性断乳期大鼠按体重随机分为低锌 (LZ)、正常锌 (NZ)和高锌 (HZ)三组 ,每组 1 0只 ,分别用锌含量不同的饲料 (2 .2 ,2 8和 1 2 8mg/kg diet)喂饲 4w,观察锌摄入量不同对大鼠红细胞膜上 Na2 /K2泵、COTS- 1、COTS- 2、Gardos和 RF等通道转运活性的影响 ;体外实验观察加入不同浓度锌 (0、5、1 0、5 0、1 0 0和 5 0 0 μmol/L Zn2 + )对人红细胞膜上这 5种阳离子转运通道活性的影响。结果 : 适宜的锌是保证 Na+ /K+ 泵、COTS- 2和 Gardos通道活性的关键因素之一 ,体内外过高或过低的锌均会抑制其离子转运功能 ,COTS- 1和 RF随锌浓度的升高转运活性增强 ,提示了其部分代偿功能。结论 : 锌对维持红细胞膜的正常离子转运功能有重要意义。
Objective: To study the effects of zinc on transport function of erythrocyte membrane. Methods: This study was conducted both in vivo and in vitro. In vivo, weanling rats were divided into three groups and fed with different zinc diets (2.2, 28 and 128 mg Zn/kg diet) for four weeks, the transport function of Na +/K + pump, COTS-1, COTS-2, Gardos and RF channels were determined. In vitro, different concentration of zinc (0,5,10,50,100 and 500 μmol Zn 2+ /L) were added into fresh human blood and the activities of the five transport channels were detected. Results: Proper zinc could keep the highest activities of Na +/K + pump, COTS-2 and Gardos channel. Too low or too high zinc decreased the transport function of these three channels and the activities of COTS-1 and RF channel were increased with the increase of zinc concentration, indicating the competitive function of these two channels. Conclusion: Zinc plays an important role in maintaining the transport function of erythrocyte membrane.
出处
《营养学报》
CAS
CSCD
北大核心
2001年第4期320-323,共4页
Acta Nutrimenta Sinica
基金
北京市自然科学基金 ( No 7972 0 2 3)
关键词
锌
红细胞膜
转运功能
微量元素
zinc
erythrocyte membrane
transport function
