摘要
目的 应用ACC-M裸鼠肺转移模型研究阿斯匹林对腺样囊性癌细胞转移的抑制作用。方法 裸鼠尾静脉注射肿瘤细胞前后各7天连续服用分别含1mg％、10mg％和50mg％阿斯匹林饮用水或消毒饮用水14天,接种肿瘤后6周处死动物,比较各实验组与对照组的裸鼠肺重量和肺转移灶面积比。结果 服用含1mg％和 50mg％阿斯匹林饮用水的实验组与对照组相比较,两项指标的差别均有显著性（P<0.05）。结论 阿斯匹林对腺样囊性癌细胞ACC-M裸鼠肺转移有抑制作用。
ve Effect of aspirin for metastases was uncertain till now. Metastatic salivary adenoid cystic carcinoma cell line (ACC - M) is highly metastatic to the lung, with a metastatic rate of 96% . So nude mice models with ACC - M cell metastases to lung were used to study on antimetastatic effect of aspirin. Methods Nude mice drank lmg% , 10mg% , 50mg% aspirin (ASA) contained water and usual water respectively from 7 days before to 7 days after injection of ACC - M cell suspension 0.2ml (106/ml) from tail vein. These mice were killed six weeks after injection of ACC - M cell. The lungs of the mice were removed, washed, weighed, and wax slide stained with hematoxylin and eosin was made for each lung. The slides were scanned by VIDAS for measuring size of lung and area of metastases, and then ratio of metastases was calculated . The value of weight of lung, ratio of metastases of lung sections between the groups of different management and group of control were statistically analyzed by SAS. Results Compared with group of control, the parameters of group 1mg%ASA and group 50mg%ASA as follow, the mean weight of lungs were 0.723g and 0.590g lower respectively, the mean ratio of metastases were 34.57% and 28.08% lower respectively, the differences were significant (P < 0.05). The parameters of group 10mg%ASA were also lower than group of control, the mean weight of lung were 0.328g lower, the mean ratio of metastases were 12.07% lower, but the differences were not significant (P >0.05). Conclusion Aspirin could reduce metastases in nude mice model with ACC - M cell metastases to lung. The number of mice might lead to statistically differences between group of lmg%ASA, 50mg%ASA and group of 10mg%ASA. Further investigation is needed to clarify [his problem.
出处
《口腔颌面外科杂志》
CAS
2001年第4期310-312,共3页
Journal of Oral and Maxillofacial Surgery
