摘要
目的：观察在慢性肾炎患者外周血中共刺激分子４－１ＢＢ的表达特点及其作用。方法：采用流式细胞仪，对３５例慢性肾炎患者外周血Ｔ淋巴细胞亚群和协同刺激分子４－１ＢＢ的表达进行了研究。结果：活动期慢性肾炎患者Ｔ淋巴细胞亚群明显失衡，表现为ＣＤ４减少，ＣＤ８增加，ＣＤ４／ＣＤ８比值显著降低。缓解期患者Ｔ淋巴细胞亚群失衡得到明显纠正。活动期慢性肾炎患者共刺激分子４－１ＢＢ显著高于正常对照组（４－１ＢＢ表达量为３０．５７±８．１２，比正常人０．７４±０．２８，Ｐ＜０．００１），缓解期患者４－１ＢＢ显著降低，而且４－１ＢＢ异常表达与ＣＤ４＋Ｔ细胞呈负相关（ｒ＝－０．８４，Ｐ＜０．０５）。结论：慢性肾炎中，Ｔ细胞活化所必需的共刺激分子４－１ＢＢ的异常表达可能为慢性肾炎发生和病变进展的免疫因素之一，为深入探讨慢性肾炎的免疫发病机理提供了重要价值。
Objective: To investigate the expression and role of costimulatory molecule 4-1BB on peripheral lymphocytes from patients with chronic nephritis. Methods: The expression of 4-1BB on T lymphocytes from 35 patients with chronic nephritis were studied by flow cytometry. Results: The T lymphocyte subset from active patients with chronic nephritis markedly imbalanced. All patients had decreased CD4+ cells and CD4 /CD8 ratio and had increased CD8+ cells. The imbalance of T lymphocyte subsets improved in remission of diseases. The 4-1BB expression on peripheral lymphocyte was muchhigherin active patients than that of normal controls(30. 57±8.12 vs 0. 74±0. 28,P<0. 001). The 4-1BB expression decreased significantly in remission patients. Moreover, negative correlation was found between the 4-1BB and CD4 + cell counts(r =-0.84, P K 0.05). Conclusion: Abnormal expression of costimulatory molecule 4-1BB essential in T cell activation may be one of immune factors in the development of chronic nephritis and warrants further study.
出处
《中国中西医结合肾病杂志》
2002年第1期20-22,共3页
Chinese Journal of Integrated Traditional and Western Nephrology
