摘要
青霉素酰化酶酶活可以利用荧光胺和6-氨基青霉烷酸(6-APA)反应加以测定。这一方法操作方便、灵敏度高。底物青霉素对反应没有干扰,其它干扰物质的影响可以通过控制pH4.0加以消除。与6-硝基3-苯乙酰氨基苯甲酸(NIPAB)方法相比,荧光胺方法可以反映酶与其真正底物青霉素之间的作用关系,在数值上,两种方法具有相关性,灵敏度高6倍。与对一二甲胺基苯甲醛(PDAB)法相比,灵敏度高3.6×10~4倍。
The reaction of 6 -aminopenicillanic acid with fluorescamine can be used to determine penicillin acylase activity. This method is simple, sensitive. Penicillin which is substrate does not react with fluorescamine. The interference of other compounds is readily controlled by keeping the PH at 4.0. Comparing with 6-nitro-3-phenylacetamido-benjoic acid method, f luorescamine method can provide information on the activity of enzyme against its usual substrate, penicillin. There is relationship between the two methods, the sensitivity of fluorescamine method is 6 times higher than6-ni tro-3-phenylacetamide-benzoic acid method, 3.6×104 times higher than p-dimethylaminobenjaldehyde method.
出处
《河北大学学报（自然科学版）》
CAS
1989年第3期90-94,共5页
Journal of Hebei University(Natural Science Edition)
关键词
青霉素酰化酶
酶活
荧光胺
测定法
Penicillin acylase, Fluorescamine, 6-Aminopenici Manic acid.
