摘要
目的 观察肾移植患者环孢素结合蛋白Ａ（ＣｙＰＡ）ｍＲＮＡ表达水平与血环孢素（ＣｓＡ）浓度和急性排斥的关系。方法 采用逆转录聚合酶链反应动态测定２３例肾移植稳定患者和１０例急性排斥患者的移植肾活检标本及外周血淋巴细胞中 ＣｙＰＡ ｍＲＮＡ的表达。结果 应用ＣｓＡ后肾功能稳定患者，ＣｙＰＡ的表达水平，在淋巴细胞（Ｐ＝０．０１３）和肾组织（Ｐ＝０．０３８）均显著上升，而急性排斥患者则无明显变化（Ｐ＞０．０５）；淋巴细胞中 ＣｙＰＡ的表达与 ＣＳＡ浓度存在低度相关性（ｒ＝０．３８４，Ｐ＝０．０４７）；急性排斥患者淋巴细胞中 ＣｙＰＡ表达水平低于肾功能稳定者（Ｐ＝０．００９）。结论 应用 ＣＳＡ预防排斥的肾移植患者淋巴细胞中ＣｙＰＡ低表达是导致排斥发生的可能因素之一。对淋巴细胞ＣｙＰＡ表达水平进行监测，并结合ＣＳＡ血浓度，有可能指导ＣＳＡ的合理应用。
Objective To observe the expression level of cyclophilin A(CyPA) mRNA in kidney tissue and peripheral blood lymphocyte of renal transplant patients, and research its relationship with acute rejection and blood CsA concentration. Methods CyPA mRNA extracted from biopsy tissue of graft kidneys and anti-coagulated blood from 23 stable patients and 10 cases of acute non-humoral rejection, was measured by reverse-transcription polymerase chain reaction. Results Enhancing CyPA expression of stable patients after using CsA occurred in peripheral lymphocytes (from 0.53±0.66 to 1.04±0.88, P=0. 013, n =21) and kidney tissues(from 0. 62±0. 16 to 0. 79 ±0. 26, P = 0. 038, n= 6), while it was not found in acute rejection patients( P > 0. 05) . CyPA expression level of lymphocytes was slightly correlated with blood CsA concentration( r=0. 384, P = 0. 047) . CyPA expression level in lymphocytes of the rejection patients decreased significantly as compared with stable patients (0. 40 ±0. 22 vs 1. 04 ±0. 88, P = 0. 009) Conclusions CyPA expression level in peripheral blood lymphocytes may be one of the factors causing acute rejection. Monitoring the CyPA expression level in lymphocytes and the blood CsA concentration may be effective for guiding the reasonable usage of CsA.
出处
《中华肾脏病杂志》
CAS
CSCD
北大核心
2002年第1期41-44,共4页
Chinese Journal of Nephrology
