摘要
目的:探讨前列腺癌病人血清PSAD 与组织原位PSA 表达的关系。方法:用免疫组化Sp 法检测前列腺癌组织中PSA,Mias 图象分析系统检测PSA 阳性目标面积比。术前一周化学发光分析法测定血清PSA 浓度及B 超测定前列腺体积。选择同期前列腺增生症病例 25例作为对照。结果:25 例前列腺癌全部为腺癌,高、中和低分化分别为5、13和7例。癌组织PSA 阳性22例,阴性 3例,表达强度同组织分化相关(P<0.05)。25 例腺癌 PSAD为0.36—>1.53(M=0.75)。22例PSA 阳性病例平均PSA 阳性目标面积比为9.2±3.3,同PSAD 呈正相关(P<0.05)。结论:前列腺癌血清PSAD 升高同癌组织中PSA 分泌细胞的数量相对增加和癌组织中蛋白酶的破坏造成PSA漏出增多有关,后者可能同原位PSA 低表达有关。
Objective: To evaluate the correlation of serum PSA density (PSAD) and expression ofPSA immunohistochemistry in patients with prostate carcinoma. Methods: The PSA level of 25 specimensof prostate carcinoma were detected by sp immunochemistry technique and positive target area ratio weremeasured using Mias image analysis system. One week before prostatectomy, the serum PSA concentra-tion was detected and prostate size was measured by ultrasonography. Twenty five cases of benign prostat-ic hypertrophy were as control. Results: All the 25 cases were adenocarcinoma with higher (5 cases),medium (13 cases) and poorly (7 cases) histological differentiation. The staining intensity of PSA immu-nohistochemistry positive (22 cases) and negative (3 cases) was correlated with histological differentiation(P<0.05) respectively. The average serum PSAD was 0.75 (0.36-->53). The average PSA posi-tive target area ratio (9.2±3.2) was proportional to the serum PSAD (r = 0.47, P<0.01), but not toPSA staining intensity and histological differentiation. Conclusion: The relative increasing of cells secret- ed PSA in per unit volume of tumor tissue was one of the causes in elevating serum PSAD. The proteolyticactivity of tumor cells promoted the leakage or escape of PSA may relate to poorly PSA expression andhigher serum PSAD.
