摘要
目的 建立高效液相色谱法测定乙肝康胶囊中芍药苷的含量。方法 采用HPLC法 ,C18柱 ,甲醇 - 0 .0 5mol·L-1磷酸氢钠溶液 -异丙醇 (40∶6 5∶2 )为流动相 ,检测波长 2 30nm ,柱温 40℃。结果 芍药苷在 0 .5～ 10 μg范围内线性关系良好 ,r =0 .9998,回收率 98.6 6 % ,RSD为 1.88%。结论 该方法比以前采用的薄层扫描法用于定量更方便、准确、可控 。
Aim To establish a RP-HPLC method for determination of Paeoniflorin in Yigankan Capsules.Method The contents of Paeoniflorin was determined by HPLC, using Spherisorb C 18 (5μm, 4.6×200mm)column, methanol-0.05mol·L -1 Natrium Dihydrogen Phosphate-Isopropanol(40∶65∶2) as the mobile phase. The detective wavelength was 230nm.Results Paeoniflorin at the concentrations of 0.5～10μg showed good linearity(r=0.9998).The average recovery was 98.66% with the relative standard deviationRSD 1.88%(n=5). Conclusion The results show that the method is simple and accurate. It can be useful in quality control of the products.
出处
《解放军药学学报》
CAS
2001年第6期332-334,共3页
Pharmaceutical Journal of Chinese People's Liberation Army
