摘要
目的 探讨甘糖酯对大鼠肝脏CuZn SOD的诱导作用。方法 RT PCR法检测甘糖酯po后的Wistar大鼠肝脏中CuZn SODmRNA的表达情况 ;亚硝酸盐法测定肝组织中CuZn SOD酶活。结果 po甘糖酯后 ,高剂量组大鼠肝组织的SODmRNA水平与对照组相比有显著的差异 (P <0 0 1) ;高剂量组大鼠肝组织CuZn SOD活力与对照组相比有非常显著的差异 (P <0 0 0 1) ,而中剂量组和低剂量组CuZn SOD活力与对照组相比有显著的差异 (P <0 0 1)。结论 甘糖酯能够诱导大鼠肝组织中CuZn SODmRNA的表达 ,并提高其酶活 。
AIM To study the effect of propylene glycol mannate sulfate (PGMS) on induction of CuZn SOD. METHODS Wistar rats were given PGMS po at different doses (0, 18 9, 37 8 and 75 6 mg·kg -1 ·d) for ten days. Then the rats were sacrificed and the total RNA was extracted from the livers. The total RNA samples were loaded on a 1% agarose gel to detect the quality of total RNA. RT PCR was applied to study the expression of CuZn SOD mRNA in rat livers. The amplified products were detected by the 1 5% agarose gel electrophoresis. Simultaneously, the CuZn SOD activities in rat liver were determined by nitrite method. RESULTS The total RNA extracted from rat livers was integrated without being decomposed by RNase. The level of CuZn SOD mRNA of the high dosage group (75 6 mg·kg -1 ·d) was higher than that of the control group (0 mg·kg -1 ·d) ( P <0 01); the CuZn SOD activities of the high dosage group were significantly higher than those of the control group ( P <0 001) and the CuZn SOD activities of the middle (37 8 mg·kg -1 ·d) and low dosage groups (18 9 mg·kg -1 ·d) were higher than those of the control group ( P <0 01). CONCLUSION PGMS can increase the CuZn SOD activities as well as CuZn SOD on mRNA level. Therefore, it is possible for PGMS to counteract Atherosclerosis (AS) by inducing the expression of CuZn SOD.
出处
《药学学报》
CAS
CSCD
北大核心
2002年第1期23-26,共4页
Acta Pharmaceutica Sinica
