摘要
目的 研究建立SEN病毒D亚型 (SENV D)聚合酶链反应 (PCR)检测方法。方法 选择SENV D开放读码框 1高度保守序列 ,设计合成 2对特异性引物 ,建立检测SENV D感染的套式PCR方法 ,对 3 2 7例无偿献血和职业献血者进行SENV感染的检测 ,并对部分PCR阳性产物进行克隆测序。结果 该方法特异性和灵敏度均较高 ,检测结果显示无偿献血人群SENV D感染率为 5 5 % ,职业献血人群为 6 7% ,两者无统计学差异。结论 我国广东部分地区无偿献血和职业献血人群中存在SENV D亚型感染 ;
Objective To establish a polymerase chain reaction (PCR) method for detection of SEN virus D (SENV D) DNA in serum Methods Two pair of primers were designed from ORF 1 of SENV D genome. The nested PCR method for SENV D was built and the specificity and sensitivity were tested, the 327 samples of the voluntary and commercial blood donors were detected by this method Results The specificity and Sensitivity of this PCR were ideal. The positive rate of SENV D DNA were 5 5% and 6 7% in voluntary and commercial blood donors. There was no significance difference ( P >0 05 ) between these two groups Conclusion There exist SENV D infection both in the voluntary and commercial blood donors in Guangdong province. The PCR could be used for the detection of SENV D DNA in serum
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2002年第1期26-28,共3页
Chinese Journal of Laboratory Medicine
