摘要
目的:观察白细胞介素2(IL-2)基因修饰对树突状细胞(DC)的生物学特征和功能的影响,探讨用IL-2基因修饰DC,增强DC介导特异性抗肿瘤免疫的机制。方法:IL－2基因修饰小鼠骨髓来源的DC后,用扫描电镜观察其表面形态的变化,FACS分析IL-2基因修饰对DC表面免疫分子表达的影响,RT-PCR方法检测DC中 IFN-γ mRNA表达。用3H-TdR掺入法检测IL-2基因修饰后,DC对同种异体T淋巴细胞的刺激作用和对肿瘤抗原的特异性提呈功能。结果:经IL-2基因修饰后,DC表面的伪足增多、变长;其表面与抗原提呈相关的免疫分子Ia、B7-1、B7-2和CD40的表达明显上调;il-2基因修饰的DC(DC-IL-2)中表达IFN-γ mRNA;CD-IL-2不但对同种异体T淋巴细胞有较强的促增殖作用,而且对肿瘤抗原的特异性提呈功能亦明显增强。结论:IL-2基因修饰DC,能促进DC的发育,上调DC表面与抗原提呈相关的免疫分子,增强了DC的生物活性。
To observe the effect of IL-2 gene modification on the character of biology and function in dendritic cells( DC) and to investigate the immune mechanism of specific anti-tumor of IL-2 gene modification in DC. Methods: DCs were prepared from mouse bone marrow and genetically modified by IL-2 adenovirus. Then observe the changes of DC morphology by scanning electro-microscopy, analyzed molecules on DC by FACS, examined the expression of IFN-γ mRNA in DC by RT-PCR.The stimulatory capacity of DC to T cells detected by MLR their capacity of antigen present were measured by 3H-TdR mix into assay. Results: After IL-2 gene modification, the morphology of DC was changed, its pseudopod was more and longer. The expression of Ia, B7-1, B7-2 and CD40 molecules was more on DC surface. The IFN-7 mRNA was expressed in the DC-EL-2 and DC-rhIL-2.DC-IL-2 could stimulate allogeneic T cells more potently and IL-2 gene-modified DC could induce more potent antigen-specific autogeneic CTL. Cooclusioo: IL-2 genetic modification can promote DC growth and up regulate their expression of membrane immune molecules that are relevant for antigen presentation of DC,and enhanced the biologic activity of the DC.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2002年第1期5-8,共4页
Chinese Journal of Immunology
基金
国家自然科学基金重点资助项目(39730420)
