摘要
作者对分离和系统鉴定的一株犬传染性喉气管炎病毒 (犬 2型腺病毒 ) SY(沈阳 )株 ,用犬肾传代细胞 MDCK进行了致弱驯化 ,每驯化 1 5代做一次初步的犬的安全性试验 ,用大剂量不同代次病毒对易感犬进行人工感染试验 ,每天观察犬的临床症状及白细胞总数 ,并于接毒后的第 5 d安乐死 ,作病理解剖学和病理组织学检查。易感犬的感染试验结果表明 ,SY株在犬肾细胞上传 1 5代时对犬的致病力已降低 ,3 0代时对犬已不引起发烧、精神沉郁和厌食等症状 ,仅引起轻度的鼻炎、中度的咽炎 ,剖检肺表现正常 ,仅见支气管淋巴结肿大 ,45代时无临床症状 ,剖检除扁桃体肿大外没有见到其它症状 ,6 0代毒已完全失去致病力 ,未见任何临床症状及病理变化。
A canine adenovirus (CAV) strain was isolated from a diarrhea dog with MDCK cell, it was demonstrated that it is a type 2 virulent strain, by systematic identification and was named SY. Since it can induce high SN antibody titer in dogs against CAV, it may be a potential vaccine strain candidate. The virulent SY strain was attenuated by serial passages in MDCK cell line. The pathogenicity of SY strain was tested by experimental infection in susceptible dogs with the virus on 15th, 13th, 45th and 60th passage lever, respectively. The result showed that the pathogenicity of SY strain decreased gradually with the increasing of passages and lost pathogenicity to dogs of the 60th passage.
出处
《中国兽药杂志》
2001年第6期7-9,共3页
Chinese Journal of Veterinary Drug
基金
军队青年基金及全军开放试验室基金课题
关键词
犬
2型腺病毒
传染性喉气管炎病毒
传代驯化
免疫原性
安全性
噬斑克隆
Canine laryngotracheitis virus (ICLV) / Canine adenovirus type 2 (CAV 2)
passage and attenuation
vaccine strain
