摘要
目的 比较低温冷冻和酒精处理的同种异体周围神经移植的疗效。方法 取 45只成年雄性SD大白鼠 ,随机分为A、B、C、D 4组。A、B、C组为实验组 ,每组 1 0只 ;D组为供体组 ,1 5只。切取双侧坐骨神经 1 5mm作为供体神经。A组的供体神经在 - 1 96℃下保存 3周 ,37℃生理盐水复温后桥接于受体一侧坐骨神经 1 0mm缺损段 ;B组用 70 %酒精浸泡 8小时后 ,桥接于受体一侧坐骨神经 1 0mm缺损段 ;C组的供体神经不经任何预处理 ,桥接于受体一侧坐骨神经 1 0mm缺损段。术后 1 7周进行电生理测定 ,光镜观察及移植异体神经中段的轴突计数。结果 A、B实验组的电生理测定及移植神经中段的轴突计数结果无显著性差异 ,光镜下的形态学变化相似。A、B组与C组 ,电生理测定与移植体中段轴突计数均有显著性差异。
Objective To evaluate the results of cryo preserved and ethanol treated peripheral allogeneic nerve grafting.?Methods Forty five male SD rats were divided randomly in four groups. A?B and C were experimental group(n=10).D was donor group(n=15). A 10 mm defect of the host sciatic nerve was made and bridged with a 1.5cm donor sciatic nerve which was cut from group D in group A ?B and C. The donor nerves were cryo preserved at -1968 hours in group B before operation. The defect of host sciatic nerves was bridged with a 15 mm donor sciatic nerve without any pretreatment in group C. Four months after operation, electrophysiological examinations and obeservation under light microscope were done, and the number of axons in the middle segment of allogenic graft was counted.?Results The results of electrophysiological examination and the count of axon did not show significant discrepancy between group A and B, the morphological changes of two experimental groups were similar. There is a significant discrepancy between group A or B and C.?Conclusion The results of cryo preserved and ethanol treated peripheral allogeneic nerve grafting are alike in 17 weeks.
出处
《创伤外科杂志》
2002年第1期31-34,共4页
Journal of Traumatic Surgery
