摘要
为探讨氯化甲基汞 (MMC)所致脑发育损伤及其与c fos表达的关系 ,采用光镜、电镜等形态学方法 ,观察了MMC对体外培养的大鼠脑神经胶质细胞的损害作用 ,并应用免疫细胞化学方法 (SP法 )观察MMC对培养神经胶质细胞c fos表达的影响。结果表明 ,体外培养的胶质细胞接触MMC后 ,出现胞浆浓缩、轴突回缩、染色质边聚、固缩等现象 ,呈凋亡的典型形态。MMC 0 0 2mmol L作用于神经胶质细胞 10min后 ,c Fos蛋白阳性细胞百分率随其后的培养时间而变化 :c Fos蛋白阳性细胞率 0 5h开始增高 ,2h达高峰 ,4～ 6h又逐渐减少。MMC 0 0 0 12 5、0 0 0 5、0 0 2、0 0 8和 0 32mmol L作用 10min后 ,0 32mmol L组的阳性率显著高于除0 0 8mmol L组外的其他各组 (P <0 0 1)。提示MMC可诱导体外培养大鼠脑神经胶质细胞c fos表达 ,并诱发其凋亡。MMC诱发神经细胞凋亡可能与c fos介导有关。
In order to explore the injury of brain development caused by methylmercuric chloride(MMC) and the relationship between the injury and c-fos expression, the damaging effects of MMC on the morphology of cultured brain glial cells derived from neonatal Wistar rat were studied by microscope and electronic microscope, and the effect of MMC on the c-fos expression of glial cells were studied by immunocytochemical method. The results showed that the characteristics of apoptosis, such as condensed cytoplasm, marginal nuclear chromatin and pyknotic nuclei were found after cultured glial cells was exposed to MMC. The expressed c-Fos protein began to increase in the nuclei of cultured glial cells at 0 5 h after MMC was added to DMEM medium for 10 min and increased markedly with time prolonged. The percentage of Fos-positive nuclei reached a peak at 2h and declined gradually from 4 to 6h. The percentage of Fos-positive glial nuclei in the group exposed to 0 32 mmol/L MMC was significantly higher than those in other groups, which were exposed to 0 00125, 0 005 and 0 02mmol/L MMC respectively ( P <0 01) at 2h after MMC was added. It is concluded that MMC can induce the expression of c-fos and the apoptosis of glial cells in vitro. The findings suggested that the apoptosis might be mediated by c-fos expression.
出处
《卫生研究》
CAS
CSCD
北大核心
2002年第1期7-9,共3页
Journal of Hygiene Research
基金
国家自然科学基金资助 (No .30 0 70 645)
