摘要
采用三亲接合法成功地将携带有发光酶基因的pTR1 0 2转入了甲基对硫磷降解菌Pseudomonassp .DLL -1菌株中。获得的接合子在液体培养、固体培养过程中以及在土壤中其标记质粒非常稳定。标记质粒并没有给受体细胞的生长、降解甲基对硫磷的能力带来严重的影响。因此 ,将标记菌株DLL -1-B用于土壤生态研究是可行的。在土壤中 ,DLL -1 -B的数量在接种后最初 2天下降幅度较小 ,随后下降速度较快 ,但当下降到 1 0 3～ 1 0 4 g- 1时趋于稳定。DLL -1 -B在水中下降速度更快 ,存活时间更短。DLL -1 -B在旱地土壤中的移动性较差 ,大多存在于 0～ 1 0cm土壤中。在水田中 ,DLL -1 -B的移动性较好 ,2周后 ,2 0～ 3 0cm土壤中测得DLL -1 -B为 2× 1 0 2 g- 1。
The plasmid pTR102 containing LuxAB gene was translated into methylparathion degrading bacterium Pseudomonas sp.DLL-1 by the tri parent conjugation method.The plasmid pTR102 in conjugator DLL-1-B was considerably stable and had no serious effect on the growth and methylparathion degrading capacity of the host.The population of DLL-1-B declined following its introduction into soil,rather slowly in the first two days and rapidly afterwards.When the population fell down to 10 3～10 4 g -1 dry soil,it leveled off.The population of DLL-1-B dropped more rapidly and its life span was much shorter in water than in soil.The mobility of DLL-1-B cells on upland soil was poor, so they usually existed in the upper 10 cm, whereas it was much better in paddy field and 2×10 2 g -1 dry soil of DLL-1-B could be detected in soil 20～30 cm in depth.
出处
《农村生态环境》
CSSCI
CSCD
北大核心
2002年第1期16-21,共6页
Rural Eco-Environment
基金
国家“九五”科技攻关项目(96-911-060-03)
