摘要
目的 建立既符合临床特征、又简便易行的标准大鼠多器官功能障碍综合征(MODS)模型。方法 用人为方法使大鼠失血 0 .5～ 0 .7ml、两后肢粉碎性骨折合并软组织挫裂形成严重复合伤 ,以诱发MODS ;参照临床标准提出大鼠的实验诊断标准 ;观察并检测 84只大鼠和16只对照大鼠在创伤 8、2 4和 48h后生化和病理变化。结果 大鼠创伤 2 4h后 ,肌酐、肌酸磷酸激酶、乳酸脱氢酶、羟丁酸脱氢酶和谷丙转氨酶浓度分别达到 ( 14 0 .3± 34 .4)mol/L、( 14 318.0±2 12 8.9)U /L、( 2 373.7± 2 74.9)U /L、( 1179.5± 2 84.9)U/L和 ( 2 98.2± 40 .6)U /L的最高值 ,与对照组相比差异具有非常显著性 (P <0 .0 1) ;48h后病理变化最明显。创伤后 2 4h的大鼠MODS处于若干脏器功能衰竭早期伴若干脏器功能受损期。结论 本研究的复制方法是成功的 。
Objective To establish a standard Sprag e Dawely rat model that wou ld not only imitate clinical features of multiple organ dysfunction syndrome (MO DS),but also be performed easily.Methods Multiple injuries involving the soft tissue,the leg bones and hemorrhage (0.5-0.7 ml per rat) in rats were made to induce MODS. A set of experiment diagnostic criteria fitting for a rat was recommended accord ing to putative clinical diagnostic criteria.Eight-four traumatic rats and 16 c ontrol rats were observed and killed at 8,24 and 48h after trauma.The biochem ical criterions were measured and the pathological changes were examined.Results Twenty-four h after trauma,the creatinine,crea tine phosphate kinase,lactic dehydrogenase,hydroxybutyric dehydrogenase and gl utamic pyruvic transaminase in the the traumatic rats were (140.3±34.4)mol/L ,(14318.0±2128.9)U/L,(2373.7±274.9)U/L,(1179.5±284.9)U/L and (298.2±40.6)U/L,respectively,which were significantly higher than in the c ontrol criterion (P<0.01).Four-eight h after trauma,the pathological chan ge was the most obvious.Twenty-four h after trauma,MODS in the rats was at the early phase of several organs failure and the phase of several organs hypofunct ion.Conclusion It was successful to establish a rat MODS model that was useful for basic study on serious trauma and therapeutic drug scr eening test.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2001年第6期506-507,共2页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目 (39670 549)
