摘要
目的 比较海藻酸 聚赖氨酸 海藻酸微囊化 (APA)大鼠胰岛和海藻酸 壳聚糖 聚乙烯乙二醇微囊化 (ACP)大鼠胰岛移植对小鼠糖尿病的治疗作用。方法 链脲霉素 2 2 0mg/kg体重腹腔内注射制作小鼠糖尿病动物模型。成模后分成APA微囊组、ACP微囊组和未微囊化胰岛组 ,每只小鼠腹腔内分别植入 2 0 0个相应胰岛 ,检测监测移植前后小鼠的血糖。结果 移植后第 1天 ,3组小鼠血糖均较移植前明显降低。未微囊化胰岛移植组小鼠术后第 2天血糖均恢复到术前水平 ;APA微囊组和ACP微囊组小鼠血糖分别维持正常 ( 5 7.0 0± 14 .61)d和 ( 41.67± 16.73)d ,此两组间差异无显著性 (P >0 .0 5 ) ,但均较未微囊化胰岛移植组明显延长 (P <0 .0 5 )。结论 ACP微囊与APA微囊一样具有免疫隔离作用 ;ACP微囊化异种胰岛移植可纠正小鼠糖尿病。
Objective To compare the effects of xenot ransplantation of Alginate-polylysine-alginate microencapsulated rat islets (A PA microencapsulated group) and alginate-chitosan-polyethyleneglycol microenca psulated rat islets (ACP microencapsulated group) in the treatment of mice diabe tes mellitus.Methods Streptozotosin 220 mg/kg intra-abdominal injecti on prepared the mice diabetic model.Three groups were assigned: naked rat islet group,APA microencapsulated group and ACP microencapsulated group.200 islets we re transplanted into the abdominal cavities of diabetic mice in each respective group.The level of blood glucose was recorded before and after transplantation.Results One day after transplantation,the level of blood glucose in all the groups was returned to normal.On the second day,the level o f blood glucose in the naked islet group was back to the pre-transplantation le vel,while that of APA microencapsulated group and ACP microencapsulated group w as kept in normal range for 57.00±14.61and 41.67±16.73 days respectively.No difference was observed between the two groups.Conclusion The ACP microcapsule could provide immune isol ation to xenoislets as the APA microcapsule;transplantation of ACP microencapsu lated rat islets could reverse the diabetic state of mice.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2001年第6期548-550,共3页
Chinese Journal of Experimental Surgery
基金
上海市科委资助项目 (9841 1 90 2 4 )
