摘要
利用质粒p16L2Ex6-3和pATH10,制备了含有HPV16L2ORF编码表位的基因克隆p16L2REx6-3。将此重组质粒转化大肠杆菌HB101,并诱导其有效表达,产生一分子量约为40KD的含有E.coli trpE的融合蛋白。Western blot分析结果表明,此蛋白可与感染HPV16的患者血清呈阳性免疫反应,提示该克隆的表达蛋白具有HPV16,的型抗原特异性。
Using the deleted plasmid p16L2Ex 6-3(insertion:human pa-pillomavirus type 16 DNA XhoII 4521-4849;vector:pA TH10),the DNA fragment(RsaI 4801-ClaI 4849)of human papillomavirus type 16 L2 ORF(HPV 16 L2 ORF)which encoded epitope was prepared.The 54 bp DNA fragment was inserted into polylinker of vector pATH 10,a expression plasmid p16L2 REx 6-3 was constructed.After transformation into E,coli HB101,the pla-staid p16L2REx 6-3 was successfully expressed as a 40KD fusion protein.Western-blot analysis showed that the expressed fusion protein appeared at expected position and could react with serum of patient infected HPV16,su-ggesting that the fusion protein contains immunoreactive epitope of HPV16 L2 and possesses HPV16 type specificity.
出处
《山东医科大学学报》
1991年第3期190-194,共5页
Acta Academiae Medicinae Shandong
基金
校中青年科学基金
关键词
乳头瘤病毒
基因克隆
质粒
Papillomaviruses
Plasmids
L2 open reading frame
Gene expression
Viral fusion proteins
