摘要
借助特异的磷酸蛋白探针 ,建立了快速检测植物类囊体膜蛋白体内磷酸化的方法 ,可以检测到光照处理的豌豆叶圆片类囊体膜中 8条磷酸化蛋白带存在 ,它们的分子质量分别为 6 5、 45、 36、 33、 30、 2 9、 2 0和10ku .进一步使用光系统Ⅱ反应中心蛋白和捕光色素复合物Ⅱ (LHCⅡ )的特异抗体 ,确定了上述磷酸化蛋白的归属 ,分别是磷酸化D1和 (或 )D2的聚合体 (6 5ku)、CP43(4 5ku)、D2 (36ku)、D1(33ku) ,LHCB1(30ku) ,LHCB2 (2 9ku)和 psbHgene产物 (10ku) ,2 0ku小肽尚不清楚其来源 .
In order to detect plant thykaloid protein phosphorylation in vivo rapidly, a new approach was introduced by using INDIA(TM) phosphorylated protein probe. Eight phosphoprotein bands were detected by this method in the thylakoid membranes isolated from pea leaf discs illuminated at 400 mu mol . m(-2) . s(-1). The molecular masses of those phosphoproteins were 65 ku, 45 ku, 36 ku, 33 ku, 30 ku, 29 ku, 20 ku and 10 ku. Respectively by using various polyclonal antibodies, those phosphoprotein bands were identified as phosphorylated D1/ phosphorylated D2 dimer, PS II core phosphoproteins, CP43 (45 ku), D2 (36 ku), D1 (33 ku) and psbH gene product (10 ku), and the light-harvesting complex (LHC II) phosphopolypeptides, LHCB1 (30 ku) and LHCB2 (29 ku). A comparison was made between this new approach with other methods of detecting phosphoprotein such as radiolabeling experiment or immunological blot using phosphothreonine antibody.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2001年第5期740-743,共4页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金 (3 9970 0 68)
教育部跨世纪优秀人才基金
优秀青年教师基金
霍英东基金资助课题~~
