摘要
应用过氧化物酶标记的链霉亲和素 (Streptavidin peroxidase简称S -P法 )免疫组织化学技术 ,用抗胰高血糖素、胃泌素、生长抑素、5 羟色胺抗体对中华鳖 (Trionyxsinensis)的胃、肠、胰内分泌细胞进行研究。结果表明 :5 HT细胞在消化道各段均有分布 ,以小肠密度最高 ,胃、大肠、直肠其次 ,食管最少。Som细胞在胃底部、幽门部和小肠各段密度较高 ,胃贲门部、大肠和直肠密度较低。Gas细胞分布于胃体部、幽门部和小肠各段 ;胃贲门部、大肠和直肠未检出Gas细胞。Glu细胞在消化道各段均未检出 。
The endocrine cells in the digestive tract and pancreas of soft shelled turtle(Trionyx sinensis) were studied by using Streptavidin peroxidase(S P), an immunohistochemical method with anti Glueagon(Glu), Gastrin( Gas ), Somatostation(Som), 5 hydroxytry ptamine(5 HT )body. The result showed that 5 HT cells were distributed in the all digestive tract, in which the density of 5 HT cells in the small intestine were highest, that in the stomachus,large intestine, rectum secondly,and that in the oesophagus lowest. The density of Som cells in the stomachus fundus,pyloricus and small intestine were higher than that in stomachus cardia, large intestine, rectum and pancreas gland. The Gas cells distributed in the stomachus corpus, pyloricus and small intestine,there were not the Gas cells in the stomachus cardia, large intestine and rectum. The Glu cells were distinguished in the pancreas gland and not in the intestine.
出处
《水产学报》
CAS
CSCD
北大核心
2001年第6期528-531,T001,共5页
Journal of Fisheries of China
关键词
中华鳖
胃肠胰系统
内分泌细胞
免疫组织化学反应
鳖
Trionyx sinensis
gastro entero pancreatic endocrine system
endocrine cells
immunohistochemical reaction.
