摘要
为探讨 SD大鼠天然抗日本血吸虫 (Schistosoma japonicum,Sj)感染的免疫学特性 ,用间接 ELISA检测正常大鼠血清 (NRS)与感染大鼠血清 (IRS)中抗成虫可溶性抗原 (AWA)及抗可溶性虫卵抗原 (SEA)的特异性抗体 ,并用酶联免疫印迹技术分析 NRS、IRS对 AWA的识别 ;将 NRS经尾静脉被动转移至昆明小鼠 ,攻击感染后第 45 d解剖冲虫 ,计算减虫率与减卵率。结果显示 NRS与 IRS中抗 AWA、抗 SEA特异性 Ig G抗体水平均高于正常昆明小鼠血清中相应的抗体水平。AWA中有两个抗原分子可被 NRS特异性识别 ,分子量为 2 72 k Da与 2 3 6 k Da;IRS则识别 AWA中分子量为2 72 k Da、2 3 6 k Da和 80 k Da的特异性条带。用 NRS被动转移后 ,与对照组相比 ,实验组小鼠的减虫率与减卵率分别为 1 9.1 0 %及 46 .42 %。故大鼠血清中存在天然抗 Sj感染的抗体 ,对
In order to explore the immunological characteristics of natural resistance to Schistosoma japonicum (Sj) infection in rat, the levels of antibodies that react to adult worm antigens (AWA) and soluble egg antigens (SEA) of Sj were assayed in normal rat's sera (NRS) and infected rat's sera (IRS) by indirect ELISA, and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) and enzyme linked immunoblotting (ELIB) methods were used to recognize AWA by NRS and IRS. Passive transfer of NRS to Kunming mice was performed to observe the acquired protection. The results showed that the levels of IgG to AWA and SEA in NRS and IRS were significantly higher than the corresponding levels observed in the uninfected mice. NRS could specifically react to two molecules of 272 kDa and 236 kDa in AWA, and IRS specifically recognized three molecules of 272 kDa, 236 kDa and 80 kDa in AWA. Compared with the control group, the reduction rates of worm burden and LEPG in test group were 19.10% and 46.42%, respectively. So natural antibodies to Sj exist in rat, which may play an important role in the resistance to Sj infection.
出处
《地方病通报》
2001年第4期25-28,共4页
Endemic Diseases Bulletin
关键词
SD大鼠
日本血吸虫
免疫特性
SD rat
Schistosoma japonicum
Immunological characteristics
