摘要
目的 了解汉坦病毒四川分离株S85 46株分子流行病学特征。方法 将特异性引物从S85 46病毒感染细胞PCR扩增的产物克隆于T载体 ,正确的克隆纯化后测序 ,应用DNASTAR软件比较分析。结果 S85 46株M片段与HTN型毒株同源性为 84.1%～ 99.7% ,而与SEO型毒株同源率仅为 70 .4%～ 70 .9% ,表明S85 46毒株属HTN型。核苷酸同源性比较显示 ,S85 46与韩国分离的 76 118株高度同源 ,而与国内一些HTN型病毒差异较大 ,而且同一地区的毒株也可以差异很大。S片段全基因序列同源性比较也支持以上的观点。
Objective To understand the molecular and epidemic characteristics of Hantavirus S85 46 isolated from Sichuan province. Methods RT PCR products were amplified from S85 46 virus infected cells, cloned into T vector, sequenced and analyzed using DNASTAR software. Results The homology of M segment of S85 46 strain and HTN type strain varied from 84.1 % 99.7 %, compared to 70.4 % 70.9 % with SEO strains. This result indicated that S85 46 strain belonged to hantavirus. The nucleotide sequence analyses indicated that S85 46 strain, LR1 strain and 76 118 strain were highly homologous. The two latter strains were isolated from Shaanxi province of China and South Korea respectively, which were far from Sichuan province of China. The nucleotide sequence analyses of S segment was similar to M segment. Conclusion The epidemic strains isolated from different regions may have been highly homologous.
出处
《中华流行病学杂志》
CAS
CSCD
北大核心
2001年第6期449-451,共3页
Chinese Journal of Epidemiology
