摘要
目的 探讨以压电传感阵列技术检测耐甲氧西林葡萄球菌的可行性。方法 收集临床 64株葡萄球菌标本 ,对其耐药标志基因mecA基因和金黄色葡萄球菌特有基因femA基因扩增后应用压电传感器阵列进行检测 ,并以PCR电泳检测作参照 ,与目前采用的常规药物敏感试验进行对比分析。结果 传感器分析与PCR电泳分析结果完全一致。而此两种方法与常规培养加药敏法比较 ,结果略有差异。所有 64株葡萄球菌中 ,2株耐甲氧西林金黄色葡萄球菌 (MRSA)的mecA为阴性 ;2株甲氧西林敏感金黄色葡萄球菌 (MSSA)的mecA为阳性。 1株耐甲氧西林凝固酶阴性葡萄球菌 (MRCNS)的mecA为阴性 ;2株甲氧西林敏感凝固酶阴性葡萄球菌 (MSCNS)的mecA为阳性。结论 以压电传感阵列技术同时检测femA基因和mecA基因 ,既可鉴定出是否为金黄色葡萄球菌 ,又可判断其耐药类型 。
Objective To investigate the feasibility of the detection of methicillin resistant Staphylococci aureus (MRSA) by piezoelectric gene sensor arrays. Methods The resistant marker gene mecA and specific gene femA of Staphylococcus aureus were detected in 64 collected strains of staphylococcus and the results were compared with those obtained by PCR and conventional antimicrobial susceptibility test. Results The results by sensor arrays were identical to those by PCR, but had some differences with those by antimicrobial susceptibility test. Among 64 strains of staphylococcus, 2 strains of MRSA had negative mecA gene, 2 strains of methicillin susceptible Staphylococci aureus (MSSA) showed positive to mecA, 1 strain of methicillin resistant coagulase negative staphylococcus (MRCNS) showed negative to mecA, and 2 strains of methicillin susceptible coagulase negative staphylococcus (MSCNS) encoded mecA gene fragment. Conclusion With the piezoelectric gene sensor arrays, mecA and femA gene can be detected at the same time, so the instrument provides a rapid diagnosis for MRSA infection.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2002年第1期20-22,共3页
Journal of Third Military Medical University
基金
国家"九五"科技攻关项目 ( 96 A2 3 0 4 0 4 )
国家自然科学基金资助面上项目 ( 39870 831 )
国家高技术计划生物技术领域青年基金资助项目 ("86 3"项目
全军"九五"医学科研计划资助面上项目( 98MO94
全军"十五"医学科研计划资助面上项目 ( 0 1MA1
