摘要
目的 :通过对几种真核细胞总RNA纯化方法的比较 ,得到一种快速、高效的纯化方法 ,以满足科研、临床、PCR等实验室快速分析的要求。方法 :采用同一种原材料 ,用三种实验方法平行实验进行结果比较。该改良方法利用特殊的Biotragents作为匀浆缓冲液 ,一步分离就能得到高纯完整的总RNA ,整个实验过程在一小时内即可完成。结果 :该方法收率、纯度及完整性并不亚于国外试剂盒及传统的纯化方法。结论 :该方法为一种快速、高效的纯化方法。
Objective: Going through the comparison of several methods for purifying eukaryotic cell total RNA,to obtain a rapid,high efficient purifying method,to meet demand for rapid assay of the research, clinical,PCR etc.Methods: Use the same kind of sample to purify the total RNA by the three different methods,after the parallel experiment to compare the Results.Using the special Biotragents as homogeneous buffer,the pure and integral total RNA can be obtained by this improved single-step method,and the whole procedure can be finished in one hour.Results: The yield,purity and integrity by this method are as same as the abroad kit and traditional methods.Conclusion: This is one kind of rapid and efficient purification method.
出处
《河南医学研究》
CAS
2001年第3期220-222,共3页
Henan Medical Research
