摘要
目的 研究化疗对卵巢癌患者细胞免疫功能的影响及其临床意义。方法 应用间接免疫荧光法、MTT比色法、ELISA检测围手术期卵巢癌患者外周血淋巴细胞(PBL)T淋巴细胞亚群、自然杀伤(NK)活性及血清可溶性白介素2受体(rIL-2R)。结果 卵巢癌患者PBL中CD3^+、CD4^+、CD4^+/CD8^+均明显低于良性肿瘤组,而Tac^+、明显升高;化疗后患者CD3^+、CD4^+明显降低,而rIL-2R、NK活性无明显改变。结论 化疗可使卵巢癌患者细胞免疫功能明显受抑,且恢复困难,需选择适当免疫治疗以提高机体自身抗瘤活性。
Objective To evaluate the effect of cisplatin-based chemotherapy on the immunity of patient with malignant ovarian tumor (MOT) . Methods T-lymphocyte subsets, natural killer (NK) activity and the levels of soluble interleukin-2 receptors (rIL-2R) were measured in 25 patients with MOT and 26 benign controls (BC) by sandwich ELISA, indirect immunofluorescence and MTT colorimetry. Results The numbers of CD3+ , CD4+ , and CD4+/CD8+ in the patients were lower than those in the control significantly, but Tac+ increased markedly (P< 0.05) . The CD3+ , CD4+ decreased remarkbly after chemotherapy, while the NK activity and rIL-2R with no significant changes. Conclusion The immune status of MOT is depressed during the course of chemotherapy, and is very difficult to improve it. Therfore suitable immunotherapy is needed.
关键词
卵巢肿瘤
化疗
细胞免疫
治疗
Malignant ovarian tumor
Chemotherapy
Cellular immunity
