摘要
目的:探讨耐多药基因(mrd-1)和耐多药相关蛋白基因(mrp)在非小细胞肺癌、癌旁和淋巴结组织中的表达关系及其对患者预后的影响.方法:采用逆转录-聚合酶链反应(RT-PCR)技术,检测了45例非小细胞肺癌、癌旁和125枚手术切除淋巴结组织的 mdr-1、mrp表达.结果:在肺癌组织中mdr-1、mrp的表达及两基因共表达的阳性率与癌旁组织比较有显著性差异(P<0.01);转移淋巴结(N1～2)的mdr-1、mrp阳性率也高于非转移淋巴结组织(N0)(P<0.01).随访发现,肺癌组织mdr-1和mrp阴性患者的术后1、2、3年生存率(89.5%,78.6%和50%)高于mdr-1和mrp阳性者(80.8%、68.8%和14.3%).结论:肺癌组织mdr-1、mrp的表达与相对应的淋巴结组织具有一致性,转移淋巴结组织的mdr-1、mrp基因表达可能与临床病理分期有关;mdr-1和mrp阴性者化疗效果好,对于阳性者,术后化疗不能提高远期生存率,而且预后不良.
ve:To investigate mdr- l,mrp gene expression and their correlation with prognosis in non -small cell lung cancer, surrounding noncancerous tissues and lymph nodes. Methods: 45 cases of primary lung cancer, surrounding noncancerous tissues and 125 lymph nodes were obtained in operation, gene expressions of mdr - 1 and mrp were measured by reverse transcription poly-merase reactions(RT - PCR). Results: Positive rates of mdr - 1 and mrp in primary foci were 57. 8 % and 55. 3 % , positive rates of two gene co- expression was 48. 9% . These rates differed significantly from those of surrounding noncancerous tissues (19%, 11.9% and 9.5% respectively;P<0.01) .The positive rates of mdr- 1, mrp and co- expression in metastatic lymph nodes were much higher than those in non - metastatic lymph nodes ( P < 0. 01) . Positive rates of mdr - 1, mrp in metastatic lymph tissues (84. 6 % , 84.6% )were significantly higher than those innon - metastatic lymph tissues while the primary faci were negative. Data of follow up examination showed that one, two, three years of survival rates in pateints with mdr- 1(and/or) mrp positive(80. 8% , 68. 8% , 14. 3% )were much lower than those in pateints with mdr - 1 and mrp negative(89. 5% , 78. 5% , 60% ). Conclusion:There is a correlation between the positive expression of primary focus of lung cancer with that of metastatic lymph tissues. Postoperative chemotherapy could not raise the long - term survival rates for pateints with mdr - 1, mrp gene expression in lung cancer tissues. Metastatic lymph tissues of patients with lung cancer may play an important role in directing postoperative chemotherapy and predicting prognosis.
出处
《临床肿瘤学杂志》
CAS
2001年第4期303-305,308,共4页
Chinese Clinical Oncology
关键词
肺癌
癌旁组织
淋巴结
基因(mdr-1
mrp)表达
预后
Lung cancer Surrounding noncancerous tissues Lymph nodes Mdr- 1 and/or mrp expression Prognosis
