摘要
建立注射用哌拉西林 /三唑巴坦的 HPCE含量测定方法。方法 :测定条件为 :运行缓冲液 :2 5 mmol/ L的磷酸硼砂溶液和 5 0 mmol/ L 的 SDS,p H9.0 ;操作电压 2 0 k V,检测波长 2 0 0 nm。结果 :测定哌拉西林的线性范围为 0 .0 1～ 0 .75 m g/ ml,日内 RSD为 0 .6 6 % ,日间 RSD为 0 .88% ;三唑巴坦的线性范围为 0 .0 2～ 1.0 mg/ ml,日内 RSD为 0 .71% ,日间 RSD为 0 .98%。哌拉西林的回收率为 99.76 % ,三唑巴坦的回收率为98.2 %。结论 :该方法简便、经济、快速 。
Objective: A HPCE method for determination of piperacillin/tazobactam for injection was developed. Methods: Measurements were carried out in a buffer solution containing 50mmol/L phosphate borax solution and 50mmol/L SDS(pH9.0), at 20kV running voltage applied to a 60cm×50μm capillary. The detection wavelength was 200nm. Results: The linear range of pineracillin is 0.01~0.75mg/ml; RSD within days is 0.66%; RSD between days is 0.9%~1.2%; The linear range of tazobactam is 0.02~1.0mg/ml, RSD within days is 0.71%, RSD between days is 0.98%. The recovery of piperacillin and tazobactam is 99.76% and 98.2% respectively. Conclusion: The method is simple, rapid, economic and accurate.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2001年第5期340-341,374,共3页
Chinese Journal of Antibiotics
