摘要
目的：应用麦芽凝集素（ＷＧＡ）沉淀法检测骨碱性磷酸酶同工酶（Ｂ－ＡＬＰ）水平，并探索Ｂ—ＡＬＰ与骨矿含量的相关性。方法：建立维生素Ｄ和钙缺乏所致的佝偻病动物模型，测定骨矿含量和血清钙、磷、２５－ＯＨＤ３、总碱性磷酸酶（Ｔ—ＡＬＰ）水平；采用ＷＧＡ沉淀法检测不同生长阶段大鼠血清中的Ｂ—ＡＬＰ活性。结果：血清Ｂ—ＡＬＰ在佝偻病组明显升高，且出现时间较Ｔ—ＡＬＰ早；Ｂ—ＡＬＰ与同期骨矿含量呈显著负相关。结论：血清Ｂ—ＡＬＰ活性是反映成骨细胞功能更为敏感和可靠的指标。
Objective: To determine the serum levels of bone alkaline phosphatase (B - ALP) by means of wheat - germ Iectin precipitation and explore correlation between B - ALP and bone mineral content. Methods: The animal model with calcium and vitamin D deficient rickets were established. Serum levels of calcium, phos phorus ,25 - OHD3 ,total ALP and bone ALP in different growth phase were assayed. Results : Bone ALP activity was significantly increased in rickets group and this increase occurred earlier than that of the total ALP. There was a significant negative correlation between bone ALP and bone mineral content. Conclusion : Serum bone ALP activity is a sensitive and reliable index in measuring osteoblastic activity.
出处
《重庆医科大学学报》
CAS
CSCD
2001年第4期402-404,共3页
Journal of Chongqing Medical University
