摘要
用免疫酶染色法(IEST)探讨了弓形虫感染的诊断价值。结果表明,人工感染弓形虫11份家兔和间接荧光抗体(IFAT)与间接血凝(IHA)抗体均阳性的34份人血清,阳性分别为11份和32份,4份阴性兔血清和108份抗体阴性人血清阳性分别为0份和2份,其敏感性为94.10%～100.00%,特异性为98.15%～100.00%.35份其它寄生虫感染者均未有交叉反应。11份阳性血清间隔半个月进行了4次重复试验,其阳性率及几何均数的倒数(GRMT)均无显著性差异。用IEST对健康体检者、不良生育史孕妇和智力低下儿童进行了测定,阳性率分别为8.46%,16.33%和28.26%,结果与IFAT无显著性差异。该法操作简单,值得在基层推广应用。
An immunoenzymic staining test (IEST) was carried out for the diagnosis of
toxoplasma infection. In 11 infected rabbit serum specimens and 35 human serum spe
cimens positive for both LHA and IFA, the positive rates detected by IEST were
100% and 94.1% respectively. Four negative rabbit serum specimens and 108 negative
human serum specimens showed the positive rates of 0 and 1.85% respectively. The
IEST test was repeated four times on 11 positive serum specimens at intervals of two
weeks, and we found no significant differences in detection rate and GRMT between
the results of these repetitions. No cross reactions were observed between the sera
from 9 patients with schistosomasis, 3 patients with paragonimiasis, 22 mice with
malaria, and 1 monkey with malaria. So we consider the IEST is very specific and
sensitive in the diagnosis of toxoplasma infections. Using IEST in screening the ser
from 130 healthy persons, 49 woman with a history of abnormal pregnancy and 46
mentally retarded children, the positive rates were 8.46%, 16.33% and 28.26%
respectively.
出处
《青岛医学院学报》
1991年第3期227-229,共3页
Acta Academiae Medicinae Qingdao Universitatis
关键词
免疫酶技术
弓形体病
常规
immunoenzyme technics
toxoplasmosis
disgnostic tests
routine
