摘要
以马粪海胆胚胎为材料,提出染色体制备方法。
The chromosomes of sea urchin are studied for breeding.Hemicentrotus pulcherriis used as experimental material in this paper.Method of the chromosomes preparation: Eggs were fertilized artificially with sperms.Early embryos were treated with colchicin and Ca-free sea water.Dissociated cells were swollen with hypotonic KCl (0.075M) The cells were fixed by mcthanol-acetic acid fixative,dried in air The chromosomes stained with Giemsa.Chromosomes numbers were analysed The 2n chromosomes number of H.pulcheri-imus have been concluded to be 46.
基金
国家自然科学基金
关键词
马类海胆
染色体
数目
胚胎细胞
Hemicentrotus pulcherrimus,chromosome number
embryo cells
diploid
