摘要
以不同剂量的紫外线 (UV)照射谷子 (Se tariaitalicacv .Shanxi)原生质体为供体与普通小麦 (Triticumaestivum)济南 1 77和 99P的原生质体用PEG法诱导融合。利用同工酶 ,RAPD和染色体分析再生的克隆及白化苗。从小麦济南 1 77与谷子融合再生的 86个克隆中 ,2 4个被鉴定为杂种克隆 ;而小麦 99P与谷子融合再生的 6 7个克隆中 ,2 7个被鉴定为杂种克隆。虽然用作融合材料的双亲培养细胞均丧失再生能力 ,但是部分来源于低剂量UV处理的杂种克隆再生了绿点、根和白化苗。证明小麦体细胞杂交中的双亲再生能力互补现象也存在于远缘族间融合组合中。
Protoplasts of millet ( Setaria italica cv.Shanxi)Irradiated with different dosages of ultraviolet light(UV)were fused with protoplasts of common wheat[ Triticum aestivum cv.Jinan 177(T177)and cv.99P(T99P)].Eighty six clones were recovered from T177(+)S( Setaria) and 24 were recognized as hybrid clones by cytological,biochemical and molecular analysis.For T99P(+)S,27 out of 67 were hybrid clones.Some clones,originated from fusion combination with mild UV treatment,were differentiated to form green spots,roots or albino seedlings,although both the praental protoplasts were non regenerable.This indicates that there is also complementary effect of regeneration capacity between fusion partners in intertribal somatic hybridization of wheat.The cause of the failure to regenerate green plants is discussed.
基金
SupportedbytheNationalNaturalScienceFoundationofChina
No .30 0 70 397andTrans CenturyTrainingProgrammeFoundationfortheTalentsbytheMinistryofEducation .
