摘要
应用重复序列REP (repetitiveextragenicpalindromic ,重复基因外回文 )和ERIC (ente robaterialrepetitiveintergenicconsensus,肠细菌重复基因间共有序列 )结合聚合酶链式反应(ERP PCR和ERIC PCR)对从新疆采集的 2 7株土著大豆根瘤菌染色体进行指纹分析 ,发现在相似水平 0 5时可基本分为两大聚类群 ,一个类群主要包括慢生型根瘤菌 ,另一类群为快生型根瘤菌 ,来自同一地区的根瘤菌株具有较高的遗传相似性。以上结果表明该技术是对大豆根瘤菌进行种群结构和遗传多样性分析的有效手段。
Repetitive(repetitive extragenic palindromic,REP,and enterobaterial repetitive intergenic consensus,ERIC) sequences in conjunction with polymerase chain reaction technique(REP and ERIC PCR) were used to fingerprint the genomes of 27 isolates of indigenous soybean rhizobia from Xinjiang.The indigenous soybean rhizobia in Xinjiang can be clustered into relative genetic similarities of approximately 0.5,of which one group mainly includes all slow-growing rhizobia,another mainly includes all fast-growing stains. REP and ERIC PCR analysis demonstrate a substantial genetic variability within members of Xinjiang indigenous soybean rhizobial populations, which reveals that genetic similarities have certain geographical correlation, and isolates from the same site have relative higher similarities.The results show that REP and ERIC PCR analysis give effective means in genetic diversity and population structure analysis of soybean rhizobia.
出处
《微生物学通报》
CAS
CSCD
北大核心
2001年第6期10-14,共5页
Microbiology China
基金
"8 6 3"子课题资助项目 (No. 96 30 2 2 )
