摘要
论述了双水相萃取在各类抗生素浓缩、分离中的应用 ,表明双水相萃取技术不仅能用于生物大分子的分离 ,而且也能用来浓缩含生物小分子物质的发酵液 .概述了双水相系统中的热力学模型和传质研究进展 .提出只有加强药物化学、生物化学、化工工艺和化学工程诸学科间的相互渗透 ,实现学科杂交 ,才是提高传统抗生素产品分离科技含量的正确方向和解决实际问题的重要途径 .对于水溶性产品 ,如抗生素、糖、糖肽等的合成 ,需在水相中进行 ,此处利用双水相系统有其明显优势 .介绍了双水相系统与固定化酶相结合的动力学控制合成头孢力新的实例 ,充分说明了新方法的特点和介绍了实验方法的梗概 .结果表明 ,双相合成远优于单相合成 ,主要体现在降低了产物头孢力新的水解程度 。
The applications of aqueous two-phase extraction in concentration and separation of various kinds of antibiotics are discussed,with good results for the separation and purification of erythromycin and penicillin G.The given technology not only can be used in the separation of biological macromolecules,but also can concentrate the broth containing small solutes of biomolecules.The progress in the thermodynamic models and study on mass transfer for aqueous two-phase systems are also presented.The integration of biochemistry, pharmaceutical chemistry, chemical technology and chemical engineering is required to increase the scientific content of traditional antibiotic production,and is also the important path for solving practical commercial problems.When the products are fully water-soluble, such as antibiotics, sugars, glycopeptides, etc, the synthesis has to be carried out in aqueous medium.In this case the use of aqueous two-phase systems could be a suitable option for continuous extraction of the products.A new approach, the combination of aqueous two-phase systems and enzymes that are covalently immobilized inside the porous structure of a support,is used in the kinetically controlled synthesis of cephalolexin.The characteristics of this new approach are explained in detail and the experimental method is also discussed.A comparison of results of the biphasic synthesis with monophasic synthesis is given.In the biphasic synthesis the degree of hydrolysis of cephalolexin decreases significantly, and its yield increases effectively.
出处
《化工学报》
EI
CAS
CSCD
北大核心
2001年第12期1039-1048,共10页
CIESC Journal
基金
国家自然科学基金重点资助项目 (No .2 9736 180 )~~
关键词
双水相萃取
抗生素
热力学
传质
动力学
合成
提取
aqueous two-phase extraction, antibiotics, thermodynamics, mass transfer,kinetically controlled synthesis
