摘要
本研究以PCR方法成功扩增并克隆了BM-1株PPV结构蛋的VP2基因的上、下游片段。通过VP2基因的序列测定,发现我国BM-1 PPV VP2基因序列与国外发表的序列(NADL-2、Kresse等)的有一定差异。
Porcine parvovirus (PPV) BM-1 strain Vp2 gene was amplified by polyme rase chain reaction with two pair of primers and two fragments. The sequences we re determined after these fragments being cloned by pGEM T vector. It was shown that the BM-1 strain PPV VP2 was different from NADL-2 in sequence analysis.
出处
《郑州牧业工程高等专科学校学报》
2001年第3期164-167,共4页
Journal of Zhengzhou College of Animal Husbandry Engineering