摘要
目的 :探讨丙型肝炎病毒 (HCV)感染的基因型及其与干扰素应答效果的关系。方法 :应用逆转录 -聚合酶链反应 (RT -PCR)技术对 2 6 9例肝炎病人血清进行HCVNS5b区基因片段扩增 ,并对 5 6例阳性PCR产物进行酶切分型 ,同时观察干扰素α - 2b对其中 48例慢性丙型肝炎的疗效。结果 :HCV单纯 1b型感染、1b与 1b混合感染及 2a型感染对干扰素的应答率分别为 14/ 2 0、2 / 2 1、5 / 7;其中完全应答分别为 9/ 2 0、1/ 2 1、4/ 7;部分应答分别为 5 / 2 0、1/ 2 1、1/ 7。 1b与 1b混合感染组同单纯 1b型及 2a型感染组比较有显著性差异 (P <0 .0 1及P <0 .0 5 ) ,而单纯 1b型感染组与 2a型感染组比较无明显差异 (P >0 .0 5 )。结论 :HCV单纯 1b型感染或 2a型感染者对干扰素敏感 ,而 1b与 1b混合感染者干扰素治疗效果极差 。
Objective: To investigate the genotypes of HCV infection in Beijing areas and the relationship between HCV genotypes and the effectiveness of inteferon (IFN) therapy.Methods:Reverse transcription polymerase chain reaction (RT-PCR) for NS5b region was used to detect HCV RNA in 269 cases.56 positive PCR products were typed by restriction endonuclease.Results:48 Chronic hepatitis C patients with different gentoypes (20 with type 1b,21 with type b/1b,7 with type 2a) were treated with recombinant interferon α-2b(IFN α 2b) for 24 weeks.The response rate of HCV genotype only 1b infection was 14/20,1b/1b co-infection was 2/21,2a infction was 5/7.Conclusion:The response rate of HCV 1b/1b was significantly lower than that of HCV only 1b or 2a.\;
出处
《河南职工医学院学报》
2001年第4期297-297,309,共2页
Journal of Henan Medical College For Staff and Workers
