摘要
从皖北盐碱地土样中分离到 0 1 1菌株 ,对其进行了菌种鉴定以及胞外碱性蛋白酶的初步研究。结果表明 :0 1 1菌株符合地衣芽孢杆菌种的特征 ,但该菌芽孢端生、孢囊膨大、中度耐盐、高度耐碱 ,可在NaCl浓度为 1 3%和pH1 1的培养基中生长 ,这些特征又不同于该种几个模式株 ,因而将 0 1 1菌株鉴定为地衣芽孢杆菌的一个亚种 ,命名为地衣芽孢杆菌砀山亚种(Bacilluslicheniformissubsp .dangshanensis)。该菌在发酵培养基中能产生较高产量的胞外碱性蛋白酶 ( 72 5u/mL)。酶的最适作用条件 :60℃、pH9.0 ,该酶在pH6.0～ 1
Strain 011 isolated from salt-soda soil in the north of Anhui province was identified,and its alkaline protease was studied.Results indicated it possesses characters common to Bacillus licheniformis,but differs from known typical strain of Bacillus licheniformis in sporange shape?spore location and tolerant degree to salt and alkalinity.The strain may grow in medium with13%NaCl and the value of pH12.Strain 011,therefore,was identified as a subspecies of B.licheniformis and designated as Bacillus licheniformis subsp.dangshanensis.Growing in fermentative medium,the strain can produce high yield alkaline protease (725u/mL),whose optimum conditions are pH 9.0 and 60℃.The enzyme is stable in the range of pH form 6-11.
出处
《微生物学通报》
CAS
CSCD
北大核心
2001年第5期20-24,共5页
Microbiology China
关键词
地衣芽孢杆菌亚种
菌种鉴定
碱性蛋白酶
Bacillus licheniformis subsp. dangshanensis
Identification
Alkaline protease
