摘要
以 Cissusquadrangularis L.为材料用不同的技术处理并比较其收缩程度、色彩保真程度以及茎附属物的保留情况。试验了 3种常规预处理方法及 2种针对肉质组织的处理方法 ,并进行 ANOVA(Analysis of variance)分析 ,结果显示 3种常规植物标本预处理方法明显不同 (P<0 .0 0 5 ) ,用 L SD(least significant difference)分析方法分析得出深冷冻法对植物标本造成的损害比汽油法 (LSD=1 .70 8)和福尔马林法 (LSD=2 .0 6 5 )要小 ;汽油法和福尔马林法处理后得到的标本质量无明显差别。用 ANOVA法分析醋酸法、纵切法得到的标本及未经任何处理的标本 ,它们的效果也有明显不同 (P<0 .0 0 1 ) ,醋酸法得到的标本比纵切法 (L SD=2 .3 71 )和未经任何处理得到的标本 (LSD=2 .1 3 8)效果更好 ,而纵切法和未经任何处理得到的标本并无明显不同 (LSD=0 .2 3 3 NS)。醋酸处理法制作小型肉质植物标本其效果得到了植物标本馆工作者的肯定。
Herbarium specimens of Cissus quadrangularis L. were prepared by different techniques and compared for shrinkage, retention of color, and persi- stence of stem appendages. The efficacy of three pretreatment techniques and two methods of handling succulent tissue were investigated. Using analysis of variance (ANOVA) there was shown to be a significant difference (P<0.005) between the three pretreatment techniques. Using the least significant diffe- rence(LSD) test, the deep- freezing technique was shown to produce less distortion in the resultant specimens than the gasoline technique (LSD=1.708) and the formalin technique (LSD=2.065). No significant difference was observed in the quality of specimens produced by the formalin and the gasoline techniques (LSD=0.357NS). ANOVA test indicated a significant difference in efficacy between the acetic acid treatment, the longitudinal sectioning treatment and untreated specimens (P<0.001). The acetic acid pretreatment was shown to be superior to the longitudinal sectioning pretreatment (LSD=2.371) and untreated specimens (LSD=2.138). No significant difference was found between the untreated specimens and those subjected to longitudinal sectioning (LSD=0.233NS). The acetic acid pretreatment technique is recommended for treating specimens of small succulents by herbarium workers.
出处
《武汉植物学研究》
CSCD
2001年第5期421-427,共7页
Journal of Wuhan Botanical Research
基金
国家自然科学基金资助项目 (项目号 :30 0 70 0 5 5
39770 0 5 5 )
