摘要
目的 从中药黄芪中提取免疫活性多糖并研究其化学结构。方法 用乙醇分级沉淀、脱蛋白、CTAB选择性沉淀、离子交换及凝胶过滤柱层析的方法从黄芪水提物提取均一多糖 ;运用凝胶过滤法确定多糖的均一性和分子量 ;运用糖组成分析、甲基化分析、Sm ith降解、IR及 1 3 CNMR等方法研究多糖的结构。结果 得到均一多糖A2 Nb。其平均分子量为 36 0 0 0 0 ,由 D-葡萄糖组成 ,主要连接方式为 α- D- (1→ 4) - Glc,在每 2 5个葡萄糖中有一个 6 -O位上的分枝。结论 从黄芪中首次分离到具有促脾细胞增殖作用的高分子量葡聚糖 A2 Nb。
Object To isolate the immunoactive polysaccharide from Astragalus mongholicus Bunge and elucidate its chemical structure Methods The polysaccharide was purified from water extracts of A mongholicus by ethanol precipitation, deproteination, selective precipitation with hexadecyltri methylammonium bromide, ion exchange and gel filtration chromatography Its homogeneity and molecular weight were estimated by gel filtration chromatography, the structure was deduced from sugar analysis, methylation analysis, Smith degradation, IR and 13 CNMR spectrophotometry Results A homogeneous polysaccharide A2Nb was obtained with a molecular mass of 360 000 , and composed of D glucose with a major linkage form of α D (1→4) glucose Side chains were found at 6 O positions once in every 25 glucose residues Conclusion A high molecular weight glucan A2Nb was obtained from A mongholicus for the first time It showed the ability of promoting the proliferation of the splenocytes of mice
出处
《中草药》
CAS
CSCD
北大核心
2001年第11期962-964,共3页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金重点资助项目 No.39730 480
