摘要
采用体外细胞培养模式 ,通过测定细胞碱式磷酸酶 (ALP)活性及细胞四甲基偶氮砜 (MTT)还原能力 ,对构成金属和合金生物材料的金属离子 (Al,Ni,Cr ,V ,Ag)的毒性进行了定量评估 ,探讨了金属离子或原子对人体组织的毒害和腐蚀作用。结果表明微量六价铬离子表现出强烈的细胞毒性。当溶液离子度高于 10 0 μmol/L时 ,钒和镍亦表现强烈毒性。银离子和三价铬离子浓度高于 5 0 0 μmol/L后表现毒性。构成老年痴呆病成因的金属铝离子的毒性具有双向性。
Cytoxicity analysis is the critical testing of evaluating biomaterials in the ASTM, ISO standards. The approach has invested in vitro the toxicity of metal ions (Al, Ni, Cr(Ⅲ), Cr(Ⅵ), V, and Ag) that construct the metallic biomaterials. By measurements of cell alkline activity (ALP) and reduction ability of cell MTT, the toxicity of prevalence metallic biomaterials has been studied, and furthermore, the poison and erosion of metal ions and atoms on human tissue are discussed. The results display that trace Cr(Ⅵ) shows serious cytotoxicity, and V as well as Ni is cytotoxicity if the ion concentration in culture medium is over 100?μmol/L. A strange phenomenon is that Ag is also cytotoxicity if the ion concentration is higher than 500?μmol/L. Al ions is biphasic in cytotoxicity. At low ion concentration (<10?μmol/L), Al ions can stimulate cell proliferation, whereas concentration is over 1?000?μmol/L, cytotoxicity increases.
出处
《中国有色金属学报》
EI
CAS
CSCD
北大核心
2001年第6期960-965,共6页
The Chinese Journal of Nonferrous Metals
关键词
生物材料
金属毒性
细胞分子生物学
biomaterials
metal toxicity
cell biological molecular
