摘要
目的 :建立虎杖注射液中白藜芦醇苷含量测定的方法。方法 :采用HypersilODS色谱柱 ,以甲醇 -水 (25∶75)为流动相 ,流速1ml/min ,检测波长290nm。结果 :白藜芦醇苷对照品线性范围0 145～2 32μg/ml ,r=0 9999 ,加样回收率98 8 % ,RSD1 15 %。结论 :用高效液相色谱法测定虎杖注射液中白藜芦醇苷含量 ,操作方便 ,结果准确 。
OBJECTIVE:To establish a HPLC method for determination of prodatin in Polygonum cuspidatum injection.M_ETHODS:The analytical column was Hypersil ODS column(100mm×2.1mm,5μm).The mobile phase consisted of methanol-water(25∶75).The flow rate was 1.0ml/min.The UV detection wavelength was 290nm.RESULTS:The linear range was 0.145～2.32μg/ml(r=0.9 999).The average recovery of prodatin was 98.8%(RSD=1.15%).CONCLUSION:The method is convenient,accurate and could be used for quality control in the production of Polygonum cuspidatum injection.
出处
《中国药房》
CAS
CSCD
2001年第8期493-494,共2页
China Pharmacy
基金
上海市卫生局科学技术发展基金资助课题 (98(B)302)
