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肉仔鸡日粮外源核苷酸营养作用初探 被引量:31

Preliminary Study on the Nutritive Role of Dietary Nucleotides to Young Chicken
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摘要 为研究日粮核苷酸 (NT) ,对肉仔鸡的营养作用 ,72只 1日龄AA肉仔鸡饲喂纯合日粮 5天以耗竭可能由母源带来的核苷酸 ,然后分成 4组 ,分别喂以添加 0 ,0 .1 % ,0 .2 %的核苷酸及 0 .5 %酵母培养物的纯合日粮。测定了 1 0日龄及 1 4日龄各组试鸡肠粘膜、肝脏中蛋白质及RNA含量 ,十二指肠绒毛高度、肠壁厚度、肠粘膜湿重等指标 ,用一次性注射大剂量3H -Tyr的方法测定了 1 2日龄各组试鸡肠粘膜蛋白质合成率。结果表明纯合日粮能显著耗竭雏鸡肠粘膜中的RNA ,纯合日粮中添加 0 .2 %NT使雏鸡肠道粘膜核酸及蛋白质含量及肝脏的核酸含量比对照皆有显著的增加 ,并能促进肠绒毛的生长及提高肠壁厚度。 To investigate the nutritive role of nucleotides (NT) to young chicken,72 0-day-old chicken were fed on purified diet (Nucleotides Free, NF) for 5 days to deplete the nucleotides. Then they were divided into four groups and fed on NF, NF+0.1% NT, NF+0.2%NT,NF+0.5% yeast culture medium respectively. The villus height, crypt depth, protein and RNA content in intestinal mucosa and liver of 10,14 day-old chicken from each group were determined. The rate of protein synthesis in intestinal mucosa were estimated by injecting large quantities of3H-labeled tyrosine into the body of 12day-old chicken. The villus height, the RNA and protein content in intestinal mucosa and the RNA content in liver from the chicken fed on NF+ 0.2% NT diet significantly increased than that of NF diet. The re sult showed that the capacity of NT synthesis in the body of young chicken is insufficient to meet their growth and development.
出处 《中国畜牧杂志》 CAS 北大核心 2001年第5期15-17,共3页 Chinese Journal of Animal Science
基金 教育部高校骨干教师资助项目
关键词 外源核苷酸 日粮 肉仔鸡 营养作用 Nucleotides Diet Chicken Nutrition role
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参考文献1

  • 1张龙翔.生物化学实验技术和方法[M].人民教育出版社,1981..

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