摘要
借碱洗脱法证明:具有致突变能力的致癌剂ｌ,２－二溴乙烷和肼在经过代谢活化后,均剂量相关地引起Ｌ１２１０细胞中ＤＮＡ的股间交联．相反,动物致癌试验表现阴性的致突变剂溴乙烷,以及通过各种致癌动物试验均显示阴性的传统常用致突变剂羟胺,在代谢活化以后的同样条件下均不能引起Ｌ１２１０细胞中ＤＮＡ的股间交联．这证明了双区理论的致癌机理观点,即致癌剂必须是双官能烷化剂,其同时引起互补碱对的交联将引起癌变．相反,股内单一碱基的变异则可能引起致突变作用．并用ＡＰＣＩ/ＳＩＭ(大气压化学电离/选择离了质谱)证明:１,２－二溴乙烷与ＤＮＡ碱基对的模型厄应,与致突实验一致主要引起Ｇ－Ｃ对的交联和发生Ｇ－Ｃ→Ａ－Ｔ突变．
By means of DNA filter elution method, it is evidenced that two carcinogens 1,2-dibro-moethane with mutagenic potential and hydrazine with weak mutagenicity, can both induce the DNA interstrand cross-link with quantity dependence in L1210 culture. However in a identical condition, two non-carcinogens with analogous structures, ethylbromide and hydroxylamine with mutagenicity and potent mutagenicity respectively, both can't induce the corresponding cross-link. These results are consistent with the mechanism of the carcinogenesis of Dai's di-region theory that the carcinogen must be a bifunctional alkylating agent, the monofunctional damage of the base with single strand can only induce the mutagenesis but not carcinogenesis.
出处
《环境化学》
CAS
CSCD
北大核心
2001年第6期537-543,共7页
Environmental Chemistry
基金
国家自然科学基金资助项目(批准号:20042001)
