摘要
利用自然病例 ,采取分组 (对照组 ,轻度腹水综合征组和重度腹水综合征组 )对比的方法 ,分别用硫代巴比妥酸法和羟胺法测定了肉鸡血清和主要组织 (心脏、肝脏、肺脏、肾脏 )丙二醛 (malondialdehyde,MDA)含量和超氧化物歧化酶 (superoxide dism utase,SOD)活性。结果表明 ,与对照组肉鸡比较 ,轻度腹水综合征组肉鸡血清和主要组织 MDA含量降低 (P<0 .0 5 )或显著降低 (P<0 .0 1) ,SOD活性升高 (P<0 .0 5 )或显著升高 (P<0 .0 1) ;而重度腹水综合征组肉鸡血清和主要组织 MDA含量升高 (P<0 .0 5 )或显著升高 (P<0 .0 1) ,SOD活性显著降低 (P<0 .0 1)。间接证明了氧自由基和氧化损伤参与了肉鸡腹水综合征的病理过程 。
The broilers were divided into three groups,including control group(C),mild ascites syndrome group(M),severe ascites syndrome group(S).Thiobarbituric acid method and hydroxylamine oxidation procedure were used respectively to determine malondialdehyde(MDA) content and superoxide dismutase(SOD) activity in serum and major tissues(heart,liver,lung and kidney) of broilers.The results showed that MDA content decreased(P<0.05) or markedly decreased(P<0.01) while SOD activity increased(P<0.05) or obviously increased(P<0.01) in serum and major tissues of mild ascites syndrome broilers as compared with the control broilers. However,in serum and major tissues of severe ascites syndrome broilers,MDA content increased(P<0.05) or markedly increased(P<0.01) while SOD activity obviously decreased(P<0.01) as compared with the control broilers.These findings indirectly proved that oxygen free radicals and oxidation injury may play an important role in the pathological process of broiler ascites syndrome and the oxygen free radicals level is relevent to the severity of broiler ascites syndrome.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2001年第6期594-596,共3页
Chinese Journal of Veterinary Science
基金
湖北省科技攻关资助项目 ( 981P0 90 7)
