摘要
利用氨基酸自动分析仪和蛋白质电泳技术,对呼和浩特地区胆石症患者胆汁做了主要氨基酸的测定及蛋白质电泳分析。报道了胆石症患者胆囊胆汁中16种氨基酸的含量;蛋白质总量中以脂肪族氨基酸含量最大;酸性氨基酸大于碱性氨基酸,两者间的差异显著。胆石症患者胆囊胆汁和肝胆汁经蛋白电泳分析结果与慢性胆囊炎患者血清蛋白电泳的变化规律相同。即慢性胆囊炎患者血清的电泳分析中白蛋白含量减少;α_2、α_2、β、γ球蛋白含量增加。此测定结果对胆石症的研究及防治提供了新的线索。
Amino acids and proteins in the biles of cholelithiasis patients are analysed with an amino acid auto-analyzer and protein electrophoretic techniques. Sixteen amino acids are quantitively analysed in the biles of cholelithiasis patients.In proteins,aliphaticamino acid makes the largest proportion.The acidic amino acids content is significantly higher than alkaline amino acid content. By protein electrophoresis analysis,the biliary protein electrophoresis changing pattern in cholelithasis patients is the same as serum protein electro- phoresis changing pattern in patients with chronic cholecystitis,that is,in patients with chronic cholecystitis,the albumin,quantity decreases;but theα_2, β,and γ giobulin quantities increase,these results provide new clues for both researching,preventing and treating cholelithiasis.
关键词
胆石症
胆汁
氨基酸
电泳
Bile
cholelithiasis
amino acid
electrophoresis
