摘要
在联帮德国奥卡河中分离到一种病毒分离物(W),通过鉴别寄主反应的测定,病毒粒子的电子显微镜观察,cDNA斑点杂交和血清学的研究,鉴定出这一病毒分离物为香石竹环斑病毒。其外壳蛋白亚基的分子量为3.8×10~4。cDNA吸印转移杂交分析表明,香石竹环斑病毒的两个RNA组份(RNA^1、RNA_2)之间没有核苷酸序列同源性。RNA_1和RNA_2分别由3.7和1.5仟碱基组成。
A virus isolate (w) has been obtained from the Oker river in W.Germany. The isolate was identified as Carnation ringspot virus (CarRSV) by means of inoculation on the test plant, electron microscopy, dot blot hybridization and serology.The molecular weight of the coat protein is 3.8×10~4. Northern blot analysis revealed that the two RNA species of the virus which consisted of c. 3.7 and 1.5kb, respectively, but without base sequence homology.
出处
《病毒学杂志》
CSCD
1989年第4期408-414,共7页
